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PRODUCT

PRODUCT

TEV protease

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제품 견적문의

TEV protease



Description

TEV protease is the site specific protease of Tabacco Etch Virus. 



TEV protease has a 7 amino acid cleavage after glutamine at its cleavage sequence of Glu-Asn-Leu-Tyr-Phe-Gln / Gly or Ser. 


Recombinant TEV protease is purified from E. coli containing a TEV protease domain of the Tabacco Etch Virus gene.

The recombinant TEV protease is 28.5 kDa in size.



  

Storage Buffer

50mM Na-P (pH 7.4), 2.5mM DTT, 1mM EDTA, 0.05% Triton X-100, 50% glycerol


Storage Temperature

Store at -70℃


Purity of TEV protease

  

  

 M: Marker protein, 1: Pepgene TEV protease (1,000 Unit/mg)

Purity of Pepgene TEV protease by SDS-PAGE under reducing conditions.

 LAL analysis of Pepgene TEV protease. 



TEV protease activity 


Result of fluorometric analysis for activity of TEV protease.

Control 0~10: TEV protease amount (ug)

Pepgene TEV protease: 25 ug





M: Marker protein, 1: W/O TEV protease, 2: Digestion TEV protease

MBP fusion protein of 116 kDa with TEV protease. Bands of 72 kDa and 44 kDa are separated.

Cleavage yield: >95%